Brain organoids hold so much promise for the future of pre-clinical research & drug discovery, but how can we access their complexity?

Visit our poster at CAOS (MSH Medical School Hamburg): functional & network activity in 5-month-old brain organoids with our 3D biointerface technology!

How does the environment shape neuronal information processing in the retina? And what does the time of the day have to do with it?

Apparently, quite a lot. For morning birds and night owls alike. Read more in this month's blog post!

A new research pulse blog is out!

This month, we look at mechanobiology, and how the physical properties of the brain impact neuronal activity.

We're at CSHL’s 3D Modeling of the Human Brain conference right now!

Meet Marco Aquila and Giacomo Sciacca at our table with our newly released 3D CMOS chip, and don't forget to attend STEMCELL Technologies' talk later!

Preprint of Benster T. et al. from University of Washington & Standford University using our BioCAM X to bring the future ever closer.

Here they recorded murine retinal ganglion cell firing to reconstruct facial images using CNNs, a first in decoding complex sensory nerve signaling.

What is the role of hybrid and biointerface technologies in biological research?

In the latest 3Brain explorer series, we take the occation of the MG-Award 2022 to reflect a bit about the past, present and future of connecting electronics to cellular networks.

We also have a brillant interview with this years winner, a project on the mysterious role of pseudo-synapses in promoting glioblastoma progression.

What are molecular mechanisms causing neuropathic pain?Great paper from De Lobo Vitoria et al. from the University of Nottingham who finds an intriguing connection between hypoxia signalling & dorsal root neuronal activation, measured by our HD-MEA.

The majority of brain cancers (gliomas) are very invasive and symptom onset often includes extraordinary changes to behaviors or dramatic side-effects like seizures. Recent studies showed that glioma cells reshape their microenvironment & form electrically functional pseudo-synapses with neurons to integrate into the brain's microcircuitry.

So one question naturally arises: What the hell is going on?Read our latest research pulse blog: Hijacking neurons to cause a seizure

We're at the Cajal workshop with our world-first Accura-3D biointerface for functional imaging of neuronal activity. The future is here, and it is 3D.

We have been working on 3 different states of a brain organoid: acute whole organoid, organoid slice, and dissociated organoids.

Last day to meet Sercan Deniz in person, but the system will be still running until October 18th with the help of Francesco Di Matteo (Max Planck Institute of Psychiatry). Come and see real data acquisition and software analysis. It will blow your mind.

We humans hurt constantly, the older we get, the more we become aware of it. Few things in life are as unpleasant as experiencing pain, which begs some foundational scientific question:

How is pain created, and why does our body make us suffer?

This simple-looking question is not without its depth, despite our intuition. Pain sensation is a complex phenomenon, as it involves the somatosensory system, the spinal cord and the brain, and can originate anywhere along this axis.

The “acute” feeling of pain that we are most familiar with is our brain’s interpretation of...

Busy day for our scientists!

Dr. Sercan Deniz is giving a talk about our 3D biointerface technology in the University of Oxford, UK, while Dr. Giacomo Sciacca doing the same in the SIBPA 2022 Conference, Italy.

Join them as our 3D tech is a game changer for brain organoids and tissue slices.

We got the chance to explain about our groundbreaking interface technology & new 3D microchip in a podcast with Labiotech. If you are a life scientist working with 3D models like organoids, organotypic tissue slices or multicellular 3D cultures, you do not want to miss this!

We cover a lot of ground, and there are many fascinating applications, so if you are working in pharma, biotech or future technologies, this 15 minute conversation would be one you'd want to listen into.

The future is here.

We are excited to finally reveal our first-in-class 3D cell-electronic interface to probe neuronal activity of brain organoids and tissue slices. There is no comparable technology out there.If you are at CSEM's organ-on-chip workshop, come talk to Sercan Deniz and Sicong YU at booth 5!

In collaboration with Swiss precision manufacturer CSEM, 3Brain AG has announced an exciting 3D microchip for their brainon- chip portfolio. This first-in-class cell-electronic interface technology will allow scientists to study the complexity of 3D cellular networks at unrivalled scale and precision – and to gain novel mechanistic insights into the inner workings of the most complex structure in the universe, the human brain.

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Neurovascular coupling research tells a story that might be vital to our wider understanding of how the brain works in physiological and pathological conditions.It might even prompt us to rethink the nature of our own thoughts.Featuring research from @Giuseppe Gagliano, @Samantha Schaeffer, @Lisa Mapelli and many others!

We're at the Cortical Development Conference 2022 this Wednesday at 13:00! We have Marco Aquila presenting about our latest developments and also our new revolutionary 3D tech. Head over to check out how our brain-on-chip tech can simplify and empower your research in exciting ways. products are made with and for researchers.We'll also be at our booth for scientific discussions and more extensive application questions. Don't be shy, we love to talk about science

How does drug abuse impact neural network remodeling during development?

Interesting paper from Veleanu et al. from (CNRS - Centre national de la recherche scientifique) using our CMOS-MEA to show neonatal PCP impacts connectivity, but causes no long-term difference in activity nor cytoarchitecture.

Fascinating new method paper from Pamplona et al., using super-resolution principles and our CMOS-MEA to map the receptive fields of mouse retina from large heterogeneous neuronal populations with high fidelity.

Can scientists model in vitro neuronal culture as a stochastic dynamical system using spatio-temporal data from HD-MEA?

Tour de force to reconstruct network connectivity & dynamics of synapses by Sun C. et. al., from the Chinese University of Hong Kong, Shatin.

Retinal ganglion cell functional classification is a key step in understanding visual processing and features extraction.Have a look at this new multimodal approach combining pharmacogenetics, immunisto chemistry and obviously large-scale activity recordings from our HD-MEAs!

HD-MEA recordings of neuronal activity by Giuseppe Gagliano et al. from the University of Pavia, Italy help uncover a non-linear frequency dependence regulating competition between NO and 20-HETE.

In this paper by Dorgau B. et al of Newcastle University, retinal organoids were tested with well-known toxic compounds showing functional impairment similar to what is obtained in vivo. Read more into how organoids can effectively be used for in vitro toxicological studies through this paper.

This latest paper by Hu X., et al. at Dr. Hayder Amin's lab in the German Center for Neurodegenerative Diseases (DZNE), with our technology on Biosensors and Bioelectronics. Here they show complex connectivity analysis of the SpatioTemporal dynamics occurring in the olfactory bulb. Therefore shedding light on the information processing and opening up the way for advanced olfactory-based biosensors and neuromorphic approaches.

3Brain is very happy to be in this year's first in-person SLAS 2022 conference. Meet us at booth 2546, right next to the Exhibitor Theater to know more about our BioCAM DupleX, HyperCAM Alpha, and the newly launched BrainWave 5.

The long awaited 3Brain - Massimo Grattarola Award 2022 has begun!
With this year's €15,000 Euro prize for the winner, we hope to continue our goal in promoting young neuroscientists' careers. We're also very excited to again be receiving the numerous great applications on project ideas, we hope to see your application soon!

3Brain is sponsoring and attending The 31st Annual Meeting of NECTAR & 16th INTR! Meet Dr. Sercan Deniz at our booth to see the BioCAM DupleX and get more info on how our HyperCAM Alpha will assist your research.

Bruno et al, and researchers from the Istituto Italiano di Tecnologia have now introduced a new, all-optical, and nongenetic method to elicit activity with high spatio-temporal resolution while simultaneously recording network activity from cardiac and neuronal cultures.

3Brain are happy sponsors of The International Workshop on Chronic Pain and Itch, organized by Instituto de Neurociencias de Alicante  on 20-22nd Oct.

Researches from the Institute of Neuroscience Milan shed light on the role of the Rho GTPase RAC1 in hippocampal circuit formation and function. Knockout of the RAC1 inhibitor ARHGAP22 led to altered synaptic structure and functional changes at the single-cell and network level. Decreased firing and bursting rates in ARHGAP22 KOs were recorded with the BioCAM system and suggest a neuronal Excitatory/Inhibitory imbalance.

3Brain AG, the world’s first company to have placed sophisticated microchips in direct contact with cells to study them, and SuSoS AG, a high-tech company specialized in functional thin film coatings, have entered a commercialization agreement and areworking together to ensure that the 3Brain’s pioneering high-resolution microelectrode arrays (HD-MEA) and CorePlate™ technologies can be adopted quickly by as many users as possible thanks to the advantages brought by SuSoS’ AziGrip4™ CELL+ surface functionalization.

The ISSCR 2021 Annual meeting begins next week on the 21st of June!
We're excited to be sharing more information with you about the HyperCAM Alpha at our booth for all those interested in 3Brain's new CorePlate™ technology for stem cells, ipsc, disease modeling, drug discovery, research, and more!

Reach out to any of us during the event, we are looking forward to sharing with you about the HyperCAM Alpha and our CorePlate™ Technology!

The success of your experiments relies highly on the generation of a robust, efficient and stable model. In this beautiful study from Cutarelli A. et al. (Università di Trento), the authors, faced with the limitations of current protocols for differentiating motor neurons from hiPSCs, have developed a new model optimized to convert hiPSCs into large quantities of functional MNs and their progenitors. Their results obtained with a 3Brain BioCAM system to assess neuronal network functionality are described in this paper.

L. Muzzi and colleagues from the Università degli Studi di Genova recently introduced a new protocol to generate electrically active cortical neurospheroids and how they perfectly pair with HD-MEAs as functional readout.

A big step towards improved cardiotoxicity assessment was made by combining laser-induced cell poration and 3Brain's CMOS HD-MEAs. In this study from Iachetta et al (Istituto Italiano di Tecnologia) the authors describe an effective and reliable method for recording intracellular signals from thousands of cardiac cells simultaneously, compatible with high throughput screenings. This technique, taking advantage of 3Brain high-resolution MEAs properties, improves the reliability and reduces the costs of cardiotoxicity assessments, also by drastically reducing the number of independent experiments required.

Register for the first Ribbon Synapses Seminar Series 2021 today!
Today's session is focusing on the inner ear (auditory, balance and spatial orientation systems): first enjoy the musical talents of Empire Wild with Ken Kubota, Mitch Lyon and Brandon Ilaw - proudly sponsored by 3Brain AG, followed by a brief introduction from our very own, Sercan Deniz.
Then learn about how we encode sounds and process them in the auditory system with Tobias Moser, from Universitätsmedizin Göttingen, and Katja Bleckmann from Carl von Ossietzky University of Oldenburg. Ruth Anne Eatock from the University of Chicago will then enter into the details of the balance system in the inner ear.

Jointly hosted by Washington University School of Medicine in St. Louis and the Collaborative Research Center of Universitätsmedizin Göttingen.

Today we'd like to announce our official collaboration with a:head bio AG on the development of 3Brain’s next generation microelectrode array (MEA): the CorePlate ™ technology.
With this collaboration we intend to improve the technology to allow for extraction of highly resolved electric signals from 3D biology in an automated, high-throughput work flow.

In this exciting paper from Norimoto et al., researchers from the Gilles Laurent lab at Max Planck Institute for Brain Research, Frankfurt am Main deciphered the function of a not so well known brain structure, the claustrum, showing how it is involved in the control of brain activity during sleep cycles. Our HD-MEAs helped measure the in vitro propagation velocity of the sharp-wave ripples (SWRs) generated in the claustrum of a reptile (the Australian bearded dragon Pogona vitticeps) during sleep.

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In memory of his pioneering work in Bioelectronics, we are honored to inaugurate the very first 3Brain - Massimo Grattarola Award on his birthday today! The Award aims to support young post doc neuroscientists into the start of their career.
Head into the page now to read the details and eligibility to apply for this research award and win 10,000€!

Visit us at #SfNConnectome21 from today till 13th at 10:00-15:30 EST: 3Brain-SFNConnectome21
We will be available for video chat, but if you're not able to catch us, we also have a link to schedule a better timing for a proper video chat: Schedule Chat
We look forward to connecting with you there!

We are thrilled to announce a great opportunity for young Post Docs. 3Brain wants to promote young neuroscientists at the beginning of their scientific journey by awarding the most inspiring project idea involving HD-MEA recordings from in-vitro models. Don’t miss the chance to win a 10,000€ award. Follow us on LinkedIn and Twitter, more details coming soon!

New paper released! Beautiful work from Diana Ixmatlahua et al (Rafael Gutiérrez team, Center for Research and Advanced Studies of the National Polytechnic Institute, (Centro de Investigación y de Estudios Avanzados del IPN) Mexico). With the simultaneous recording of thousands of cells on a 3Brain HD-MEA, they discovered that the well-known glutamatergic mossy fiber synapse of the hippocampus was hiding silent but ready-to-use, pH-sensitive, electrical synapses.

We would like to provide an update on 3Brain AG, especially after the press conference held by the Federal Council of Switzerland last Friday.

Due to the events of COVID-19 in Switzerland. We would like to inform and ensure everyone on 3Brain's flow of operations as we will continue to stick to our commitments to our customers. Events will be ensured as far as we can and we will update all personnel by email for any changes ahead of time to avoid inconveniences on your behalf.

If you do have any inquiries, please feel free to get in contact directly with us at: info@3brain.com

One of our first conferences into 2020!

Showing the true potentials of HD-MEAs as to how we bring CMOS technology together with MEAs. Displaying not only the BioCAM DupleX, but also the upcoming BioCAM MultipleX!

As written by JNeurosci, "Electrical coupling between different types of ganglion cells exists in mammalian retinae, highlighting complexity of cross-talk between distinct CNS neural pathways."

This publication shows that with the right tools, researchers still redefine studies that have been taught for well over 20 years!

This new publication shows an intriguing approach to infere the intrinsic state of neurons starting from large-scale high-density MEA recordings.Applied to 3Brain data it has been made possible to determine the role of the refractory state in spatiotemporal dynamics of developing neonatal retinal waves.

We attended the event for BraYn 2019 this year. Meeting many young and innovative neuroscientists of the field this year. We hope we brought forth some inspiration for technological advancements in the Neuroscience field, and hopefully be a contribution to the research, experiments, and successes of the young generations today.

On Nov 12 for the CSEM Business Day, with the theme of Deep Tech, many great special guests such as Dava Newman, Nasa former Deputy Administrator, and Lukas Viglietti, President at SwissApollo, spoke at the event. Our CEO (Mauro Gandolfo) also presented that day on the topic of Brain On Chip.

It has been our pleasure being here with many professionals in this year's SFN! We'll definitely see you guys again next year, but in the meantime, follow us here to get updates or ask us questions on our upcoming microelectrode array (MEA) developments.

We will be attending and exhibiting at the SFN 2019 this year. We will be present at our booth (No.1368) with our BioCAM DupleX and an upcoming product to the BioCAM line which we call MultipleX. We shall be bringing more information to the booth on how our multiwell system platform highly differentiates itself within the field of electrophysiology.

With united inputs on a publication, we've combined our system with other setups. In this particular paper, it is demonstrated how a ferrule-top micro-indentation system is used to measure visco-elastic properties of the retina along with electrophysiological activity.

We attended the 2019 SPS (Safety Pharmacology Society) in Barcelona this year. Mainly providing details at our booth about the BioCAM DupleX.  Information such as regarding how it functions in applications with neuronal cultures, organoids, brain slices, stem cells, safety in toxicology, and drug discovery.

Spontaneous activity of an immature dissociated hippocampal cultures at 11DIVs.

Despite the young age, the culture exhibits a sustained activity with strong spike events on many electrodes. Sporadic network bursts show the onset of synchronous activity.

We were at the European Retina Meeting 2019 in Helsinki this year. Having our booth displaying the BiocamX, BioCAM DupleX and our new Accura Chip to all those innovative individuals who are interested in new concepts and technology to assist them in the field of research behind retinas.

It has been a pleasure to be here at the workshop held at the University of Edinburgh. We've attended with many other professionals to discuss about the next generation of MEAs for electrophysiology. Demonstrations on BrainWave4 was made to show it's possible contribution into the future along with our new BioCAM DupleX.

Introducing our fully bi-directional microelectrode array platform, the BioCAM DupleX. Through 15 years of research and development, 3Brain has created a state-of-the-art microelectrode array with more cutting-edge functions based on the latest generation of Khíron chips.

We will be at the Stem Cells & Organoids in Development & Disease Symposium in Amsterdam on February 20-22. Meet us at booth #50 to see how our cutting-edge microelectrode array technology boosts research on organoids and stem cells.

We're bringing our advanced microelectrode array technology to the City of Lights this February 18th and 19th. Our Roadshow in Paris is an incredible opportunity to discover BioCAM X, our MEA probes and latest BrainWave 4 software and see how they meet your research needs. To find out more, send an email to info@3brain.com.

BrainWave 4 offers a new way to collect, analyze and export electrophysiological data gathered using 3Brain's microelectrode array (MEA) platforms. With this feature-rich software, you can filter the data, detect and sort spikes in activity, analyze local field potentials (LPFs), and much more. Find out how you can download BrainWave 4 and choose the right license to advance your research in drug discovery, brain organoids, neuronal networks and the retina.

Neurotoxicity and the accumulation of extracellular amyloid-beta1–42 (Aβ) peptides are associated with the development of Alzheimer’s disease (AD) and correlate with neuronal activity and network dysfunctions, ultimately leading to cellular death. However, research on neurodegenerative diseases is hampered by the paucity of reliable readouts and experimental models to study such functional decline from an early onset and to test rescue strategies within networks at cellular resolution. To overcome this important obstacle, in Amin et al., authors used 3Brain's CMOS-chips with 4096-electrodes to model AD on rat hippocampal cell culture.

In a recent paper published on Cell Reports, Hilgen and colleagues presented a "method for automated spike sorting for recordings with high-density, large-scale multielectrode arrays". Exploiting the dense sampling of single neurons by multiple electrodes, an efficient, low-dimensional representation of detected spikes consisting of estimated spatial spike locations and dominant spike shape features is exploited for fast and reliable clustering into single units. Millions of events can be sorted in minutes, and the method is parallelized and scales better than quadratically with the number of detected spikes. Performance is demonstrated using recordings with a 4,096-channel array and validated using anatomical imaging, optogenetic stimulation, and model-based quality control. Their approach demonstrates that it is feasible to reliably isolate the activity of up to thousands of neurons and that dense, multi-channel probes substantially aid reliable spike sorting.

How human neural networks fire spiking activity and responds to electrical stimuli when they coupled to a dense multi-electrode arrays for up to 3 months