Optimized software for recording and analyzing HD electrophysiological data
BrainWave 4 pairs with 3Brain's CMOS-based high density multielectrode arrays (CMOS-MEAs) to visualize, record, and analyze electrophysiological cell activity in real-time.
Customizable display tools show this dense data in a user-friendly video interface. Pixels on the color-coded activity map correspond with the 4,096 electrodes with changes in color representing activity spikes/voltage traces.
To showcase and share results, these specially configured activity maps can be exported directly into videos.
Sophisticated algorithms make it possible to observe spatial distribution and activity patterns and detect isolated signals.
BrainWave 4 features advanced visualization settings that further the study of organoids, animal- and human-derived stem cells, cellular (neuron or cardiomyocyte) networks, brain slices and the retina. Superimposing an image (or several) of the tissue culture onto the color-coded activity map makes it easy to locate and observe cell activity detected by the HD-MEAs.
Thanks to the wide array of selection tools on BrainWave 4, there are an infinite number of ways to organize and analyze HD electrophysiological data.
Selected channels can also be grouped and compared with other groups, facilitating data analysis, creating graphs, and exporting files. Raw CMOS-MEA data can also be filtered by a variety of statistics such as mean firing rate, where only electrodes within a given range are included.
BrainWave 4 performs both real-time and off-line analyses on the HD electrophysiological data collected on 4,096 channels. After spike detection or local field potential (LFP) detection (available on-line and off-line) is configured and run, a myriad of calculations are possible, such as:
The new BrainWave 4 software offers a host of compression strategies to simplify data management and save space on your hard disk. Dynamic lossy compression lets you choose the percentage of relevant signal to be preserved (signal masking) and the percentage of noise to be discarded (noise blanking).
BrainWave 4 stores all your data using an HDF5 hierarchical data format (hdfgroup.org). This open-source, cross-platform, large data storage solution is supported by most data analysis platforms like Python, Matlab, Scilab, Octave and R. Additionally, charts and images generated from raw data can be exported as Microsoft Excel spreadsheets.
Brainwave 4 offers a series of add-ons designed to meet researchers' needs for specific experiments and data analyses.
This intuitive and easy-to-use settings panel manages the internal stimulator in our BioCAM products. With the click of a button, send electrical impulses through the HD-MEA stimulating channels or an external stimulating electrode connected to the IO Connector Box for BioCAM X and BioCAM DupleX.
EVOS is a complete tool to record and analyze evoked activity in brain slices in real-time and off-line. It's suited for Long Term Potentiation (LTP), Long Term Depression (LTD) and experimental protocols which involve recording evoked local field potentials in brain tissues. With this add-on, you can set up an ad-hoc stimulation protocol, record raw data in specific intervals to save hard drive space and extract signal features like peak amplitude, delay and signal energy in real-time.
* License includes: (S)tandard, (P)remium, and (E)nterprise
Uncompressed raw data recording
Real-time spike and LFP detection
Online data filtering
Spike and LFP data recording
Compressed raw data recording
Memory buffer extension to record past events
Off-line data filtering
Spike and LFP detection on single files
Average and mean firing rates
Export raw data
Export video basic version
Export video full version
Channel grouping by statistic
Superimpose images on MEA layers
Box plot statistics
Export chart data in Excel format
Multiple file batch analysis
* Online features are free when purchasing a BioCAM acquisition system.