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The 3Brain -
Massimo Grattarola Award
Application Info

Brain Organoids and 3D Neuronal Cultures

Generate electrophysiology maps for precise brain imaging in vitro. Record electrophysiological activity in brain spheroids, brain organoids and retinal organoids.

Models

  • Brain/CorticalOrganoids

  • Retinal Organoids

  • Spheroids

  • Scaffold/matrix-based 3D cultures

Advantages

In vitro Brain Organoids or Spheroids reproduce key brain in-vivo organization features as cell–cell 3-dimensional interactions or tissue-specific architecture. This makes models a great resource for assessing the efficacy of drugs in brain disease studies and for toxicological and compound screening. 3Brain’s single-well and multiwell CorePlate™ can measure functional activity from brain organoids at an unprecedented high spatial resolution.

Advantages of 3Brain technology with brain organoids are:

  • Label-free functional monitoring of whole organoids with single-cell spiking activity resolution.

  • Improved statistical significance measuring from thousands of neurons simultaneously

  • Multiple sample recordings: 3Brain’s single-well CorePlate™ has a sensing area of up to 25mm², allowing to record multiple spheroids in a single experiment.

Click here to know how the technology works

Tools & Features

Integrated spike and field detection algorithms within the BrainWave software - 3Brain
  • Integrated spike and field detection algorithms

    BrainWave software detects, extracts and classifies spikes or field potentials from organoids.

Superimpose and Focus on BrainWave - 3Brain
  • Superimpose and Focus

    BrainWave integrates advanced tools to superimpose images of your samples on the activity map and zoom on it. This feature allows you to record the selected region of interestor to focus on a specific area of activity for advanced analysis.

Automated statistical analysis of network parameters - 3Brain
  • Automated statistical analysis of network parameters

    The all-in-one BrainWave softwarehelps the user to quickly obtain results from an experimental session in asimple and intuitive way.

Ability to design your experimental session through the BrainWave software - 3Brain
  • Design your experimental session

    The BrainWave  intuitive graphical user interface gives the user full control of the experimental designto rapidly obtain results from an experimental session.

Feel free to get in contact with our neuroscientists team for details

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Customer experience

A collection of reviews from our customers

The Muotri lab uses 3Brain high-resolution multi electrode arrays to understand how human neural networks are formed during development, and become altered in several neurological conditions.
It is a pleasure to work with the 3Brain team, they are both highly skilled experts and friendly.

Dr. Fred Jordan, Co-CEO

FinalSpark
Fred Jordan's 3Brain review
The BioCAM offers, for the first time, recordings at a scale that allow validation of models of neuronal population activity. This is a major step that will drive, in turn, the development of theories in neuroscience.
Our laboratory works on brain experimental recordings and modeling on multiple scales, from molecular/cellular to whole brain in animals and humans. The use of BioCAM X integrates perfectly into the workflow as it allows a unique set of measurements on distributed network activities.
The BioCAM has revolutionized the way we look at population activity in the retina. With its overall large size and spatial resolution, it allows us to study population behaviour at unprecedented spatial and temporal detail.
We engineer a novel plasticity neuroelectronic platform using CMOS-MEA, nano-microtechnology and computational tools to decipher the molecular and functional plasticity simultaneously in a large-scale circuit/network in health and disease.
I have conducted a wide range of brain slices as well as neuron culture experiments in 3Brain's HD MEA system. Their technology is beyond expectation in terms of sensitivity, durability, and high STN ratio.
3Brain has impressed me with the quality of its products. The BioCAM DupleX has been the perfect platform to aid our drug discovery efforts at Xenon, and the personal one-on-one assistance continues to exceed my expectations.
The HD MEAs allow us to perform accurate cardiotoxicity assessments on hiPS cardiomyocytes by applying a novel laser technology for recording high-quality action potentials from thousands of cardiac cells simultaneously.

Selected Publications

View all  
Organoids
Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light

(paper)

Celiker, C., Weissova, K., Cerna, K.A., Oppelt, J., Dorgau, B., Gambin, F.M., Sebestikova, J., Lako, M., Sernagor, E., Liskova, P. and Barta, T.,

Iscience (2023) DOI: 10.1016/j.isci.2023.107237

Organoids
Human-derived cortical neurospheroids coupled to passive, high-density and 3D MEAs: a valid platform for functional tests

(paper)

Muzzi L., Di Lisa D., Falappa M., Pepe S., Maccione A., Pastorino L., Martinoia S. and Frega M.

Bioengineering (2023) DOI: 10.3390/bioengineering10040449

Organoids
Incorporating microglia-like cells in human induced pluripotent stem cell-derived retinal organoids

(paper)

Chichagova V., Georgiou M., Carter M., Dorgau B., Hilgen G., Collin J., Queen R., Chung G., Ajeian J., Moya-Molina M., Kustermann S., Pognan F., Hewitt P., Schmitt M., Sernagor E., Armstrong L. and Lako M.

Journal of Cellular and Molecular Medicine (2023). DOI: 10.1111/jcmm.17670

Do you want to know more?
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