Our microchips, both in the form of single-well HD-MEAs and when integrated in CorePlate™ multiwells, can gather high-resolution electrophysiological data from large networks of cultured neurons. Experimenters can browse the activity of spontaneous and chemically-induced spatio-temporal patterns, watch the neural network’s response to stimuli and even study complex network topology.
200 ms of spontaneous electrophysiological activity of hippocampal cell cultures (22 days in vitro) showing sustained burst propagations over the whole network.
100 ms of spontaneous electrophysiological activity of hippocampal cell cultures (15 days in vitro) seeded at a low cellular density in order to resolve cellular activity (~4,000 neurons).
Recording of electrically evoked responses (100 ms) of a cultured hippocampal network (days in vitro) using two (A, B) different on-chip stimulating electrodes. Even responses below 3 ms can be recorded without electrically induced artifacts.
Data provided courtesy of Dr. Luca Berdondini's team at NetS3 Lab, Istituto Italiano di Tecnologia, Italy.
Two different examples (A and B) of different activity patterns recorded from the same hippocampal culture (22 DIV) from a 2-day old post-natal rat.
Spiking activity recorded from primary cortical neuronal culture at DIV 25 with a 3D scaffolding.
Data provided courtesy of Mariateresa Tedesco, from Prof. Sergio Martinoia's lab, DIBRIS, University of Genova, Italy.
Field potentials and multi-unit activities can be sensed with unprecedented spatio temporal detail with both our HD-MEA (microelectrode array) and CorePlate™ powered 6-well devices .The large field of view enables assays on large tissue portions, such as rat cortico-hippocampal slices or brain organoids, to be carried out.
Cerebellar slice recording from a mouse (21 days old), showing spontaneous electrophysiological activity in Purkinje cells.
Data provided courtesy of Prof. Egidio D'Angelo's team (in particular: Dr. Lisa Mapelli, Dr. Teresa Soda, Dr. Francesca Locatelli) from the University of Pavia.
Functional imaging of distinct inter-ictal (I-IC) events obtained by superimposing the neuronal activity, here recorded from a cortico-hippocampal slice on a 3Brain high-resolution microchip, with a micrograph of the slice itself.
Data provided courtesy of Dr. Enrico Ferrea and Dr. Benfenati’s team, NBT / Synaptic Neuroscience, Istituto Italiano di Tecnologia, Italy (adapted from Ferrea et al. 2012).
Recording of a rat hippocampal slice (14 days old) during spontaneous activity (A) and after perfusion with 4-Aminopyridine (B immediately after; C: 15 min after perfusion).
Data provided courtesy of Franco Ortiz, from Prof. Rafael Gutiérrez's lab, Centro de Investigación y Estudios Avanzados del Insituto Politecnico Nacional, Mexico.
Recording of hippocampal activation from two different mice, respectively 21 (A) and 27 (B) post-natal days, under perfusion with 4-Amino-Pyridine.
Data provided courtesy of Dr. Alessandro Maccione and Dr. Luca Berdondini's team, NetS3 Lab, Istituto Italiano di Tecnologia, Italy.
Our microchips integrated in high-density microelectrode arrays (HD-MEAs) and in CorePlates™ multiwells are ideal for investigating explanted retinas, thanks to their large-scale sensing areas (up to 5.1 x 5.1 mm²) that feature a temporal resolution which outperforms traditional imaging techniques.
Recording of spontaneous electrophysiological activity from the ganglion cell layer in the Cone Rod Homeobox knockout mouse retina, a model of photoreceptor dystrophy. Dystrophic retinas are characterized by pathological, strong spontaneous bursting and oscillations in the ganglion cell layer. In this example, bursts are generated in cell bodies and propagate along axons converging toward the optic disc.
Spontaneous waves recorded from the ganglion cell layer in a P11 mouse retina. The activity becomes stronger in the presence of the GABA-A receptor antagonist bicuculline (10 µM) and furosemide (100 µM), a blocker of the potassium-chloride co-transporter KCC2.
Light-elicited ganglion cell responses of a mouse retina (P113) showing the activity of retinal ON ganglion cells after onset of a full field light stimulus (2.8 cd*s/m2). Immediately after stimulus onset, ON transient ganglion cell types respond to the light followed by more ON sustained ganglion cell types. The optic disc is in the middle, the dorsal side at the bottom and the ventral side at the top.
Retinal ganglion cell axonal responses to flickering (1Hz) checkerboard stimulus under dark mesopic conditions. The recording clearly shows propagating impulses along axonal bundles (P113 days mouse).
Data provided courtesy of Dr. Evelyne Sernagor and Dr. Gerrit Hilgen, The Institute of Neuroscience (ION), NewCastle upon Tyne, UK.
Recording of light-elicited activity of salamander retinal ganglion cells.
Data provided courtesy of Dr. Tim Gollisch and Dr. Vidhyasankar Krishnamoorthy, University Medical Center Göttingen, Dept. of Ophthalmology, Göttingen, Germany.
