BioCAM DupleX

A new frontier in functional imaging

Introducing a fully bi-directional microelectrode array platform for 2D and 3D in vitro electrophysiology

Overview

In vitro electrophysiology reimagined

BioCAM DupleX is a state-of-the-art microelectrode array, also known as a multielectrode array (MEA), platform for powerful in vitro functional imaging.

Its bidirectional capabilities allow researchers to simultaneously record high-density electrophysiological data at 18kHz and induce electrical stimulation at any of the 4,096 microelectrodes.

In addition to this micrometer-precise electrical stimulation, active and passive light shielding allow the use of optogenetic tools without affecting chip functionality.

This advanced platform works with both classical planar as well as innovative 3D CMOS MEA technology.

The BioCAM DupleX will utilize our latest generation of Khíron chips, which feature 4,096 µNeedle electrodes to penetrate structured brain tissues like slices, organoids, spheroids and obtain signals from inside your biological models, thus dramatically increasing the quality of your data samples.

Our journey to perfection

1 product, 15 years of R&D

BioCAM DupleX is the result of 15 years of research and development, a culmination of our experience in CMOS microelectrode array (MEA) devices, electrophysiological signals, data processing and biological sample conditions. It is electrophysiology at its best.

Electrophysiology in 3D

An ideal match: BioCAM DupleX and Khíron

The BioCAM DupleX is the first MEA plaftorm of its kind, able to power 3D high-density CMOS-MEA chips.

With the Khíron ASIC family developed by 3Brain, the BioCAM DupleX can simultaneously record and stimulate from all 4,096 microelectrodes structured in the penetrating µNeedle grid.

These revolutionary µNeedle electrodes access information inside all kinds of tissues like slices, organoids, spheroids and scaffolded 3D cultures without damaging them, making the BioCAM DupleX the only platform for in-tissue 3D functional imaging.

Electrophysiology revolutionized

Complex electrophysiology setups, where the wiring of a single electrode to the amplifier can significantly change the noise level and impair the experiment, or where the interpretation of the signals coming from your setup is restricted to electrophysiological experts, are now a distant memory.

BioCAM DupleX makes setup as easy as possible, automating important settings, optimizing noise and making electrophysiology accessible to everyone. Complex electrophysiological traces are turned into functional images, producing results similar to those you can obtain with a microscope: easy to understand qualitatively and quick to process for quantitative results.

Sensing and actuation with 4,096 electrodes

Bidirectional platform

When paired with Khíron MEA probes, the BioCAM DupleX can use any of the recording sites to release an electrical stimulation, thus making it a fully bidirectional platform.

Coupled with BrainWave, a unique integrated software for recording/stimulation and real-time processing, BioCAM DupleX allows researchers to easily setup complex experiments to study functional connectivity, information processing and complex behaviors like learning or memory in neuronal networks - all without the need for external stimulating electrodes and third-party electronics.

Touch button and LED interface

Precision at your fingertips

After inserting a microelectrode array (MEA) chip into the BioCAM DupleX, the motorized locking mechanism activates with a simple press of the lock button to enable precise contact aligment. The system automatically recognizes the MEA chip and is ready to acquire data.


A convenient set of LED lights shows when the BioCAM DupleX is powered on, whether the MEA chip is locked in and when the system is recording.

A close-up view

Integrated reference

The BioCAM DupleX features a microelectrode array (MEA) chip integrated reference electrode that simplifies the experimental procedure, reducing the risk of contamination, interference noise and accidental spill out.

Under the case

Hover on the BioCAM DupleX to discover more.

Real-time processing

Advanced FPGA control to denoise, filter and compress high-density data

Magnetic surfaces

All the surfaces around the MEA chip are magnetic for easy setup with other equipment, such as perfusion holders

Temperature control

Integrated heating and cooling system

Integrated stimulator

2 independently programmable stimulator channels (1 voltage and 1 current)

Fast locking system

A motorized solution to reliably lock and unlock your microelectrode array (MEA) chips

Robust external case

Crafted from aluminium to shield from electromagnetic and mechanical noise

The BioCAM user experience

Attention to detail

Here at 3Brain, we pay attention to each and every detail because we know how much they count in the success of your experiments.

From the intricate electronic ciruitry and the mechanics of the device to the state-of-art material chosen for the case and the optimized user interface, even down to the product packaging, everything has been designed to give you a unique experience and to help you concentrate on what matters most: your results.

Perfect fit in any setup

Compact design for maximum impact

The BioCAM DupleX microelectrode array, also known as a multielectrode array (MEA), platform is packed with features to enrich your research. At the same time, its small footprint makes for easy integration with instruments like microscope and perfusion or patch-clamp systems.

W 230mm/9.5''
D 180mm/7.08''
H 39mm/1.53''

Tech specs

Main controller

Computational core Intel®'s Arria® 10 FPGA 13Gbps, 2GB DDR4, and ARM® dual-core Cortex™-A9 1.5 GHz
Data resolution 12 bit
Number of simultaneous recording channels 4096
Sampling frequency (full-array) 20 kHz/electrode
Region-of-interest 1 - 4 independent subsets of electrodes (up to 64kHz sampling frequency)
Temperature control Active heating and cooling system
Data interface USB 3.1 Type-C
Ground reference On-chip integrated
HD-MEA compatibility Prime, Arena, Stimulo, Accura
3D HD-MEA compatibility Accura 3D (firmware upgrade required)
Control software compatibility BrainWave 4 or higher
Inputs Two analog inputs (-3.3 V to 3.3 V) or triggers (LV-TTL)

Stimulation module

Integrated stimulation module Yes
Real-time stimulation controller Yes
Internal (on-chip) stimulation sites 4096 (only with HD-MEA Accura)
External stimulation sites 4 differential channels (accessible with optional connector box)
Current stimulus generator Yes
Number of channels 1 (only for external stimulation sites)
Maximum amplitude +/- 1 mA
Amplitude resolution 10 µA
Time resolution 10 µs
Voltage stimulus generator Yes
Number of channels 1 (only for internal stimulation sites)
Maximum amplitude 10 V (peak-to-peak)
Amplitude resolution 200 µV
Time resolution 10 µs

Physical specs

Body material Anodized aluminum and stainless steel
Locking mechanism Motorized
Protection from liquid spill over Anti spill barrier v. 2
Dimensions (W x D x H) 180 x 230 x 42 mm / 7.09 x 9.05 x 1.65 inches
Weight 2 Kg / 4.4 pounds